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NORDISK PSYKOLOGISK LITTERATUR - Taylor & Francis

479. 2.4×104. –. FITC. 494. 520.

Af 488 extinction coefficient

390. 479. 2.4×104. –. FITC. 494.

The computation of natural radiation in architecture - GUPEA

used have extinction coefficient between 5000 and 200.000 cm-1 M-1. The quantum yield of a molecule is the number of photons it emits per photon absorbed. It is correlated to the extinction coefficient and a higher extinction coefficient gives a higher quantum yield.

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For IgGs, we find that labeling with 4–9 moles of Alexa Fluor® 488 dye per mole of antibody is optimal. Storing and Handling the Conjugates Store the labeled protein—which will be in PBS, pH 7.2, containing ~2 mM sodium azide—at Fluorophore label: Alexa Fluor® 488 dye Reactive group: maleimide Reactivity: thiol groups on proteins and ligands, oligonucleotide thiophosphates Ex/Em of the conjugate: 493/516 nm Extinction coefficient: 72,000 cm-1M-1 Spectrally similar dyes: Fluorescein (FITC), Cy®2 Molecular weight: 720.66 Typical Conjugation Reaction 21 rows The extinction coefficient (ε) of DyLight 488 isEasily find your required extinction coefficient (ε) with AAT Bioquest's Extinction Coefficient Database. Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak. 20 rows Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. Yes, you can use the extinction coefficient of eGFP at 488nm. Be sure to measure at the right pH (find in literature for which pH you cited value is valid).
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-1.65. 488 ÅF 1985; SS-EN ISO 11885.

where 71,000 cm-1M is the approximate molar extinction coefficient of the Alexa Fluor® 488 dye at 494 nm. For IgGs, we find that labeling with 4–9 moles of Alexa Fluor® 488 dye per mole of antibody is optimal. Storing and Handling the Conjugates Store the labeled protein—which will be in PBS, pH 7.2, containing ~2 mM sodium azide—at Fluorophore label: Alexa Fluor® 488 dye Reactive group: maleimide Reactivity: thiol groups on proteins and ligands, oligonucleotide thiophosphates Ex/Em of the conjugate: 493/516 nm Extinction coefficient: 72,000 cm-1M-1 Spectrally similar dyes: Fluorescein (FITC), Cy®2 Molecular weight: 720.66 Typical Conjugation Reaction 21 rows The extinction coefficient (ε) of DyLight 488 isEasily find your required extinction coefficient (ε) with AAT Bioquest's Extinction Coefficient Database. Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.
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M cm−1 Alexa Fluor 4.. Alexa Fluor 4.. Alexa Fluor 5. If you do not have accurate extinction coefficients and quantum yields, please make the measurements or arrange Fluor 555 dye was similar to the Cy3 dye, and the Alexa Fluor 647 dye was Extinction coefficients for all unconjugated dyes in methanol length of 488 nm. Alexa Fluor 488 is a very good choice for flow cytometry.